《植物生理学报》 2017, 53(6): 979-988

花生MAPKKK基因的鉴定和盐胁迫响应分析

孔祥远¹, 秦贵龙¹, 尹秀波², 乔利仙¹, 王晶珊¹, 隋炯明^1,*, 李运祥^3,*

¹青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 山东青岛266109; ²山东省农业技术推广总站, 济南250013; ³泰山学院生物与酿酒工程学院, 山东泰安271000

通信作者：隋炯明；E-mail: suijiongming@163.com; tsuyxli@163.com

摘　要：

MAPKKK (mitogen-activated protein kinase kinase kinase)在植物抗逆胁迫中起重要作用。为分析花生中MAPKKK基因的情况, 本试验对耐盐花生(Arachis hypogaea)突变体(S2)和对照(S4)的叶片进行RNA测序分析, 筛选出了19个具有完整开放阅读框的MAPKKK基因。它们位于花生A组野生种的8条染色体上, 不同成员的氨基酸残基个数、外显子数目差异很大。聚类分析表明19个花生MAPKKK基因能聚到MEKK和Raf两个亚家族。我们利用S2和S4构建了盐胁迫处理前后的表达谱, 筛选出4个受盐胁迫诱导表达的MAPKKK基因, 分别属于MEKK亚家族(3个)和Raf亚家族(1个)。该研究为花生MAPKKK基因的功能研究和利用提供参考。

关键词：花生; RNA测序; MAPKKK; 盐胁迫

收稿：2016-12-01   修定：2017-04-07

资助：国家自然科学基金(31571705、31301356和31471542)、山东省科技发展计划(2014GNC110002)和泰安市科技局项目(20132088)。

Identification and salinity stress-responsive analysis of MAPKKK genes in peanut

KONG Xiang-Yuan¹, QIN Gui-Long¹, YIN Xiu-Bo², QIAO Li-Xian¹, WANG Jing-Shan¹, SUI Jiong-Ming^1,*, LI Yun-Xiang^3,*

¹College of Life Sciences, Qingdao Agricultural University/Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong 266109, China; ²The Agricultural Technology Extension Station of Shandong Province, Jinan 250013, China; ³College of Biology and Brewing Engineering, Taishan College, Taian, Shandong 271000, China

Corresponding author: SUI Jiong-Ming; E-mail: suijiongming@163.com; tsuyxli@163.com

Abstract:

Mitogen-activated protein kinase kinase kinase (MAPKKK) plays an important role in resisting biotic and abiotic stresses in plants. In order to analyze MAPKKK genes in peanut (Arachis hypogaea), RNA sequencing (RNA-Seq) was conducted with the use of leaves of a salinity tolerant peanut mutant (S2) and its control (S4) as materials in this experiment. Nineteen MAPKKK genes with complete open reading frame, distributed throughout 8 chromosomes of A genome, were screened. The numbers of their amino acid residues and of exons were significantly different among the different members. Clustering analysis showed that these 19 MAPKKK genes were distributed to MEKK and Raf subgroups. To analyze the response to salinity stress of these 19 MAPKKK genes, digital gene expression profiles were constructed with S2 and S4 before and after salinity treatment. The results show that 4 MAPKKK genes belonging to MEKK (3) and Raf (1) subgroups were responsive to salinity stress. This study could provide basis for functional research and the application of MAPKKK genes in peanut.

Key words: peanut; RNA sequencing; MAPKKK; salinity stress